RFID-enabled real-time manufacturing execution system for discrete manufacturing: Software design and implementation

Discrete manufacturing (DM) refers to produce products in non-sequential processes so as to respond to market and customer requirements quickly under limited lead-time. However, in shop-floor management, DM companies usually confront challenges such as information gaps between different manufacturing units, slow responsiveness to customer changes, and poor visualization. The main reasons are lacking of efficient manufacturing data collection manners and software to support shop-floor management. This paper introduces an RFID-enabled real-time manufacturing execution system (RT-MES) for improving DM shop-floor management level in the perspective of illustrating the RT-MES software design and implementation. Several contributions from this paper are significant. First, a framework of RFID-enabled RT-MES is proposed, which is generic and helpful for enterprise information system (EIS) construction. Second, a plug-universal database-aided design (PUDAD) concept and its realization are elaborated, which could reduce RT-MES development and implementation cycle. Third, an interface middleware is reported to enable RT-MES real-time intercommunication with other EISs such as SAP ERP. Fourth, a real-life case study describes how RT-MES to enhance a typical DM firm's shop-floor management, which can be referenced by other DM companies when they initiate and implement RFID-enabled solutions. © 2011 IEEE.published_or_final_versionThe 2011 IEEE International Conference on Networking, Sensing and Control (ICNSC 2011), Delft, the Netherlands, 11-13 April 2011. In Proceedings of ICNSC, 2011, p. 311-31

On the Successful Encapsulation of Water Droplets into Oil Droplets

Compound water-in-oil microdroplets can serve as microreactors in chemical and biological analyses. The inkjet printing is a useful technique to generate compound microdroplets by droplet impact. To understand the underlying physics during the droplet impact, a combined experimental and numerical study is carried out. The effect of spreading condition, impact velocity, and oil viscosity are investigated. The balance of the tripe-line among the three interfaces dominates primarily the stable morphology of the compound droplet. Reducing oil viscosity can reduce the required impact velocity. High impact velocity is necessary to reduce the side-slipping of the water droplet

A comparative study on the mineralogy, chemical speciation, and combustion behavior of toxic elements of coal beneficiation products

The huge demand for high-quality coal in China has resulted in increased generation of preparation plant wastes of various properties. A series of beneficiation products collected from a preparation plant were characterized to understand their petrographic and mineralogical characteristics, as well as thermochemical and trace element behavior during combustion. The minerals in the Luling preparation plant wastes from Huaibei coalfield mainly included kaolinite and quartz, with minor calcite, ankerite, pyrite, illite, chalcopyrite, albite, K-feldspar, anatase/rutile, and iron-oxide minerals. Massive clay lumps of terrigenous origin, cleat-infilling carbonate, and pyrite of epigenetic origin were prone to be enriched in the middlings and coal gangue. Minor or trace heavy minerals also reported to the preparation plant wastes. The contents of low-density density vitrinite and liptinite were enhanced in the clean coal, while inertinite-maceral group were enriched in the middlings. The modes of occurrences of toxic elements differed between raw coal and the waste products; and their transformation behavior during heavy medium separation is largely controlled by clay minerals (V, Cr, Co, Sb, and Pb), carbonate minerals (Co and Pb), sulfide minerals (As, Cu, Ni, Cd, and Zn) and organic matters (V, Cr, Se, and Cu). Three groups were classified based on the volatile ratio (Vr) of toxic elements. Group 1 includes the highly volatile element Se with Vr &gt; 85%; Group 2 contained elements As, Pb, Zn, Cd and Sb, with the Vr in the range of 20&ndash;85% and V, Cr, Co, Ni and Cu with Vr less than 20% were placed into Group 3. Thermal reactivity of coal inferred from the combustion profiles could be significantly improved after coal beneficiation, whereas the increased inorganic components probably inhibited the thermal chemical reaction of wastes.<br style="line-height: normal; text-align: -webkit-auto; text-size-adjust: auto;" /

Effects of cyclooxygenase-1 and -2 gene disruption on Helicobacter pylori-induced gastric inflammation

Background. Cyclooxygenases (COXs) play important roles in inflammation and carcinogenesis. The present study aimed to determine the effects of COX-1 and COX-2 gene disruption on Helicobacter pylori-induced gastric inflammation. Methods. Wild-type (WT), COX-1 and COX-2 heterozygous (COX-1 +/- and COX-2 +/-), and homozygous COX-deficient (COX-1 -/- and COX-2 -/-) mice were inoculated with H. pylori strain TN2 and killed after 24 weeks of infection. Uninfected WT and COX-deficient mice were used as controls. Levels of gastric mucosal inflammation, epithelial cell proliferation and apoptosis, and cytokine expression were determined. Results. COX deficiency facilitated H. pylori-induced gastritis. In the presence of H. pylori infection, apoptosis was increased in both WT and COX-deficient mice, whereas cell proliferation was increased in WT and COX-1-deficient, but not in COX-2-deficient, mice. Tumor necrosis factor (TNF)-α and interleukin-10 mRNA expression was elevated in H. pylori-infected mice, but only TNF-α mRNA expression was further increased by COX deficiency. Prostaglandin E 2 levels were increased in infected WT and COX-2-deficient mice but were at very low levels in infected COX-1-deficient mice. Leukotriene (LT) B 4 and LTC 4 levels were increased to a similar extent in infected WT and COX-deficient mice. Conclusions. COX deficiency enhances H. pylori-induced gastritis, probably via TNF-α expression. COX-2, but not COX-1, deficiency suppresses H. pylori-induced cell proliferation. © 2006 by the Infectious Diseases Society of America. All rights reserved.published_or_final_versio

Leaderless genes in bacteria: clue to the evolution of translation initiation mechanisms in prokaryotes

<p>Abstract</p> <p>Background</p> <p>Shine-Dalgarno (SD) signal has long been viewed as the dominant translation initiation signal in prokaryotes. Recently, leaderless genes, which lack 5'-untranslated regions (5'-UTR) on their mRNAs, have been shown abundant in archaea. However, current large-scale <it>in silico </it>analyses on initiation mechanisms in bacteria are mainly based on the SD-led initiation way, other than the leaderless one. The study of leaderless genes in bacteria remains open, which causes uncertain understanding of translation initiation mechanisms for prokaryotes.</p> <p>Results</p> <p>Here, we study signals in translation initiation regions of all genes over 953 bacterial and 72 archaeal genomes, then make an effort to construct an evolutionary scenario in view of leaderless genes in bacteria. With an algorithm designed to identify multi-signal in upstream regions of genes for a genome, we classify all genes into SD-led, TA-led and atypical genes according to the category of the most probable signal in their upstream sequences. Particularly, occurrence of TA-like signals about 10 bp upstream to translation initiation site (TIS) in bacteria most probably means leaderless genes.</p> <p>Conclusions</p> <p>Our analysis reveals that leaderless genes are totally widespread, although not dominant, in a variety of bacteria. Especially for <it>Actinobacteria </it>and <it>Deinococcus-Thermus</it>, more than twenty percent of genes are leaderless. Analyzed in closely related bacterial genomes, our results imply that the change of translation initiation mechanisms, which happens between the genes deriving from a common ancestor, is linearly dependent on the phylogenetic relationship. Analysis on the macroevolution of leaderless genes further shows that the proportion of leaderless genes in bacteria has a decreasing trend in evolution.</p

Additive effect of LRP8/APOER2 R952Q variant to APOE ε2/ε3/ε4 genotype in modulating apolipoprotein E concentration and the risk of myocardial infarction: a case-control study

BACKGROUND: The R952Q variant in the low density lipoprotein receptor-related protein 8 (LRP8)/apolipoprotein E receptor 2 (ApoER2) gene has been recently associated with familial and premature myocardial infarction (MI) by means of genome-wide linkage scan/association studies. We were interested in the possible interaction of the R952Q variant with another established cardiovascular genetic risk factor belonging to the same pathway, namely apolipoprotein E (APOE) epsilon2/epsilon3/epsilon4 genotype, in modulating apolipoprotein E (ApoE) plasma levels and risk of MI. METHODS: In the Italian cohort used to confirm the association of the R952Q variant with MI, we assessed lipid profile, apolipoprotein concentrations, and APOE epsilon2/epsilon3/epsilon4 genotype. Complete data were available for a total of 681 subjects in a case-control setting (287 controls and 394 patients with MI). RESULTS: Plasma ApoE levels decreased progressively across R952Q genotypes (mean levels +/- SD = RR: 0.045 +/- 0.020, RQ: 0.044 +/- 0.014, QQ: 0.040 +/- 0.008 g/l; P for trend = 0.047). Combination with APOE genotypes revealed an additive effect on ApoE levels, with the highest level observed in RR/non-carriers of the E4 allele (0.046 +/- 0.021 g/l), and the lowest level in QQ/E4 carriers (0.035 +/- 0.009 g/l; P for trend = 0.010). QQ/E4 was also the combined genotype with the most significant association with MI (OR 3.88 with 95\%CI 1.08-13.9 as compared with RR/non-carriers E4). CONCLUSION: Our data suggest that LRP8 R952Q variant may have an additive effect to APOE epsilon2/epsilon3/epsilon4 genotype in determining ApoE concentrations and risk of MI in an Italian population

Domain-Based Identification and Analysis of Glutamate Receptor Ion Channels and Their Relatives in Prokaryotes

Voltage-gated and ligand-gated ion channels are used in eukaryotic organisms for the purpose of electrochemical signaling. There are prokaryotic homologues to major eukaryotic channels of these sorts, including voltage-gated sodium, potassium, and calcium channels, Ach-receptor and glutamate-receptor channels. The prokaryotic homologues have been less well characterized functionally than their eukaryotic counterparts. In this study we identify likely prokaryotic functional counterparts of eukaryotic glutamate receptor channels by comprehensive analysis of the prokaryotic sequences in the context of known functional domains present in the eukaryotic members of this family. In particular, we searched the nonredundant protein database for all proteins containing the following motif: the two sections of the extracellular glutamate binding domain flanking two transmembrane helices. We discovered 100 prokaryotic sequences containing this motif, with a wide variety of functional annotations. Two groups within this family have the same topology as eukaryotic glutamate receptor channels. Group 1 has a potassium-like selectivity filter. Group 2 is most closely related to eukaryotic glutamate receptor channels. We present analysis of the functional domain architecture for the group of 100, a putative phylogenetic tree, comparison of the protein phylogeny with the corresponding species phylogeny, consideration of the distribution of these proteins among classes of prokaryotes, and orthologous relationships between prokaryotic and human glutamate receptor channels. We introduce a construct called the Evolutionary Domain Network, which represents a putative pathway of domain rearrangements underlying the domain composition of present channels. We believe that scientists interested in ion channels in general, and ligand-gated ion channels in particular, will be interested in this work. The work should also be of interest to bioinformatics researchers who are interested in the use of functional domain-based analysis in evolutionary and functional discovery

Differential regulation of myeloid leukemias by the bone marrow microenvironment

Like their normal hematopoietic stem cell counterparts, leukemia stem cells (LSC) in chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) are presumed to reside in specific niches in the bone marrow microenvironment (BMM)1, and may be the cause of relapse following chemotherapy.2 Targeting the niche is a novel strategy to eliminate persistent and drug-resistant LSC. CD443,4 and IL-65 have been implicated previously in the LSC niche. Transforming growth factor (TGF)-β1 is released during bone remodeling6 and plays a role in maintenance of CML LSCs7, but a role for TGF-β1 from the BMM has not been defined. Here, we show that alteration of the BMM by osteoblastic cell-specific activation of the parathyroid hormone (PTH) receptor8,9 attenuates BCR-ABL1-induced CML-like myeloproliferative neoplasia (MPN)10 but enhances MLL-AF9-induced AML11 in mouse transplantation models, possibly through opposing effects of increased TGF-β1 on the respective LSC. PTH treatment caused a 15-fold decrease in LSCs in wildtype mice with CML-like MPN, and reduced engraftment of immune deficient mice with primary human CML cells. These results demonstrate that LSC niches in chronic and acute myeloid leukemias are distinct, and suggest that modulation of the BMM by PTH may be a feasible strategy to reduce LSC, a prerequisite for the cure of CML